Evaluation of the Periodontal Regenerative Properties of Patterned Human Periodontal Ligament Stem Cell Sheets

Joong-Hyun Kim et al.

Jeong-Ho Yun, Chonbuk National University of Korea

Link To Paper

Abstract: The aim of this study was to determine the effects of patterned human periodontal ligament stem cell (hPDLSC) sheets fabricated using a thermoresponsive substratum. Results – The hPDLSCs showed rapid monolayer formation on various surface pattern widths. Compared to cell sheets grown on flat surfaces, there were no significant differences in cell attachment and growth on the nanopatterned substratum. However, the patterned hPDLSC sheets showed higher periodontal ligamentogenesis-related gene expression in early stages than the unpatterned cell sheets. Conclusions – This experiment confirmed that patterned cell sheets provide flexibility in designing hPDLSC sheets, and that these stem cell sheets may be candidates for application in periodontal regenerative therapy.

Keywords: Extracellular matrix, Periodontal ligament, Regeneration, Stem cells, Tissue engineering

Methods: In this study, we fabricated patterned hPDLSC sheets using nanotopographical cues to modulate the alignment of the cell sheet.

Cell Type(s): Human Periodontal Ligament Stem cells