The integration of nanotopographic patterns with hiPSC-CM culture have been shown to induce structural and phenotypic development of hiPSC-CM monolayers by enhancing cytoskeletal organization and cellular alignment and providing greater sensitivity to conduction-blocking compounds and overall dose-response relationships (Carson et al., 2016; Smith et al., 2020). The NanoSurface platform by Curi Bio (Seattle, WA) can be used as a versatile platform for enhancing the structural development of cells and improving their physiological relevance in disease modeling. Culturing hiPSC-CMs on nanopatterned 25-mm coverslips (e.g., Curi Bio ANFSCS25) placed in a 6-well plate may facilitate the development of CPVT-specific cell morphology characteristics related to dyadic ultrastructure and contractile function through hiPSC-CM maturation.
Originally Published in: Current Protocols (2021) (Link to Paper)
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