What protocols do you have for the use of the dishes. In the literature, several different seeding densities were used. What do you recommend?

As with any cultureware, optimal seeding density on ANFS can vary according to cell type and the purpose of the experiment. However, we recommend researchers use exactly the same cell density that they use for normal 35mm dishes, 6-well dishes and so on. Protocol information is provided on the ANFS product pages.